منابع مشابه
3D-Culture System for Heart Regeneration and Cardiac Medicine
3D cultures have gained attention in the field of regenerative medicine for their usefulness as in vitro model of solid tissues. Bottom-up technology to generate artificial tissues or organs is prospective and an attractive approach that will expand as the field of regenerative medicine becomes more translational. We have characterized c-kit positive cardiac stem cells after long-term cultures ...
متن کاملNovel 3D culture system for study of cardiac myocyte development.
Insufficient myocardial repair after pathological processes contributes to cardiovascular disease, which is a major health concern. Understanding the molecular mechanisms that regulate the proliferation and differentiation of cardiac myocytes will aid in designing therapies for myocardial repair. Models are needed to delineate these molecular mechanisms. Here we report the development of a mode...
متن کاملCardiac Muscle Cells in Culture
The effect of amiodarone on the expression of myosin isoforms and the growth of neonatal rat cardiac muscle cells in culture was studied by native gel electrophoresis, assays of DNA and protein synthesis, and electron microscopy. Cardiac myocytes exposed to amiodarone in the absence of triiodothyronine (T3) showed predominant V1. When cardiac myocytes were exposed to amiodarone in the presence ...
متن کاملStem cells cardiac differentiation in 3D systems.
Cardiac regeneration requires a complex cascade of events. Stem cell therapy and tissue engineering are newly emerging tools with promising potential for recover or replace of damaged cardiac tissue. There are many factors, most of them still no clarified, that limit the effectiveness of these treatments and their translation to the clinic. Cells should graft, survive and functionally integrate...
متن کاملCulture and Assay Systems Used for 3D Cell Culture
Mammalian cell culture has served as an invaluable tool in cell biology for several decades. Mono layers of adherent cells grown on flat and rigid two-dimensional (2D) substrates, such as polysty rene or glass, have evolved as the mainstay in conventional cell culture systems. Two-dimensional cell culture studies have played a pivotal role in furthering our understanding of developmental biol...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research
سال: 2016
ISSN: 0167-4889
DOI: 10.1016/j.bbamcr.2015.11.036